Bioresilience 2.0: reconsidering preparedness and response adaptability against high uncertainty in surging and repeated bioevents
DOI:
https://doi.org/10.66585/Keywords:
One health, zoonoses, host species barrier, global catastrophic biorisks, decontamination, bioresilience, biosurvelliance, microbiomeAbstract
Suboptimal management, declining host immunity and fitness, and increased pathogen diversity, evolution, and emergence constitute, as a whole, a worrisome biosecurity perspective, especially through the One Health lens. This article aims to increase systemic resilience, robustness, and effectiveness against bioevents affecting the entirety of potential hosts, including humans, animals, plants, and microbes, in all relevant environments: urban, rural, industrial, hospital, and wildlife. Bioresilience 2.0 integrates better governance with improved cognition and diverse relevant technologies to make preparedness and response relevant, adequate, sustainable, and affordable. The main message of this conceptual review is that emerging bioscience technologies, empowered by new or improved concepts and operating procedures, implemented through hardware derived from other fields, and governed optimally, may result in more effective preparedness and response. As practical implications, less systemic liability and increased readiness are expected, with dispersed, multiple, sustainable formats achievable through affordable commercial support technologies and means, coupled to integrative approaches that stress flexibility, procedural and technical synergies, and are informed by integrative thinking across sectors and by innovative, coordinated planning by the respective implicated agencies, which would guide surveillance and intervention.
References
1. Kambouris ME. The concept of humanome and the microbiomic dimension. In: Kambouris ME, editor. Genomics in Biosecurity. 1st ed. London: Elsevier Academic Press; 2021. p. 15–28.
2. Koblentz GD. The de novo synthesis of horsepox virus: Implications for biosecurity and recommendations for preventing the reemergence of smallpox. Health Secur. 2017;15(6):620–628. https://doi.org/10.1089/hs.2017.0061
3. Thèves C, Crubézy E, Biagini P. History of smallpox and its spread in human populations. In: Drancourt M, Raoult D, editors. Paleomicrobiology of Humans. Washington, DC: ASM Press; 2016. p. 161–172. https://doi.org/10.1128/9781555819170.ch16
4. Dowdle WR. The principles of disease elimination and eradication. Bull World Health Organ. 1998;76(2):22–25.
5. Jezek Z, Khodakevich LN, Wickett JF. Smallpox and its post-eradication surveillance. Bull World Health Organ. 1987;65(4):425–434.
6. Roeder P, Mariner J, Kock R. Rinderpest: The veterinary perspective on eradication. Philos Trans R Soc Lond B Biol Sci. 2013;368(1623):20120139. https://doi.org/10.1098/rstb.2012.013
9
7. Velegraki A. Panmicrobial microarrays. In: Kambouris ME, Velegraki A, editors. Microbiomics: Dimensions, applications, and translational implications of human and environmental microbiome research. 1st ed. London: Elsevier Academic Press; 2020. p. 95–119. https://doi.org/10.1016/B978-0-12-816664-2.00006-2
8. Toussaint A, Chandler M. Prokaryote genome fluidity: Toward a system approach of the mobilome. In: van Helden J, Toussaint A, Thieffry D, editors. Bacterial Molecular Networks: Methods and Protocols. New York: Humana Press; 2012. p. 57–80. https://doi.or
g/10.1007/978-1-61779-361-5_4
9. McNamara T, Platonov A, Elleman T, Gresham L. The human-animal interface and zoonotic threats: The Russian Federation approach. Biosecur Bioterror. 2013;11(3):185–195. https://doi.org
/10.1089/bsp.2013.0054
10. Magouras I, Brookes VJ, Jori F, Martin A, Pfeiffer DU, Dürr S. Emerging zoonotic diseases: Should we rethink the animal–human interface? Front Vet Sci. 2020;7:582743. https://doi.org/10.3389/f
vets.2020.582743
11. Beckham TR, Brake DA, Fine JB. Strengthening One Health through investments in agricultural preparedness. Health Secur. 2018;16(2):92–107. https://doi.org/10.1089/hs.2017.0069
12. Švábenská E. Systems for detection and identification of biological aerosols. Def Sci J. 2012;62(6):404–411. https://doi.org/10.14429/
dsj.62.1251
13. Millett P, Snyder-Beattie A. Existential risk and cost-effective biosecurity. Health Secur. 2017;15(4):373–383. https://doi.org/10.
1089/hs.2017.0028
14. Kambouris ME. Innovative aspects of the updated (meta)biothreat and the AI leverage. In: Kambouris ME, editor. Preparing for the Meta-Biothreat: Disruptive Biosecurity. Cham: Springer Nature Switzerland; 2026. p. 87–103. https://doi.org/10.1007/978-3-032-25690-4_5
15. Schoch-Spana M, Cicero A, Adalja A, Gronvall G, Kirk Sell T, Meyer D, et al. Global catastrophic biological risks: Toward a working definition. Health Secur. 2017;15(4):323–328. https://doi.org/10.1
089/hs.2017.0038
16. Gibson-Fall F. Military responses to COVID-19, emerging trends in global civil-military engagements. Rev Int Stud. 2021;47(2):155–170. https://doi.org/10.1017/S0260210521000048
17. Rhodes J, Fisher MC. Global epidemiology of emerging Candida auris. Curr Opin Microbiol. 2019;52:84–89. https://doi.org/10.101
6/j.mib.2019.05.008
18. Huang SH, Triche T, Jong AY. Infectomics: Genomics and proteomics of microbial infections. Funct Integr Genomics. 2002;1(6):331–344. https://doi.org/10.1007/s10142-002-0048-4
19. Kambouris ME, Manoussopoulos Y, Kantzanou M, Velegraki A, Gaitanis G, Arabatzis M, et al. Rebooting bioresilience: A multi-OMICS approach to tackle global catastrophic biological risks and next-generation biothreats. OMICS. 2018;22(1):35–51. https://doi.
org/10.1089/omi.2017.0185
20. Kambouris ME, Manoussopoulos Y, Velegraki A, Patrinos GP. Disruptive biosecurity: A new concept for preparedness and response to the emerging total biothreat landscape. Comp Strateg. 2026;45(2):163–195. https://doi.org/10.1080/01495933.2025.25
65586
21. Jackson RJ, Ramsay AJ, Christensen CD, Beaton S, Hall DF, Ramshaw IA. Expression of mouse interleukin-4 by a recombinant ectromelia virus suppresses cytolytic lymphocyte responses and overcomes genetic resistance to mousepox. J Virol. 2001;75(3):1205–1210. https://doi.org/10.1128/JVI.75.3.1205-1
210.2001
22. Dance A. The shifting sands of “gain-of-function” research. Nature. 2021;598(7882):554–557. https://doi.org/10.1038/d41586-021-02903-x
23. Kambouris ME. Global catastrophic biological risks in the post-COVID-19 world: Time to act is now. OMICS. 2023;27(4):153–170. https://doi.org/10.1089/omi.2022.0178
24. Partap M, Verma V, Thakur M, Bhargava B. Designing of future ornamental crops: A biotechnological driven perspective. Hortic Res. 2023;10(11):uhad192. https://doi.org/10.1093/hr/uhad192
25. Marrero J, Coto O, Goldmann S, Graupner T, Schippers A. Recovery of nickel and cobalt from laterite tailings by reductive dissolution under aerobic conditions using Acidithiobacillus species. Environ Sci Technol. 2015;49(11):6674–6682. https://doi.org/10.1021/acs.est.
5b00944
26. Anderson JA, Ellsworth PC, Faria JC, Head GP, Owen MDK, Pilcher CD, et al. Genetically engineered crops: Importance of diversified integrated pest management for agricultural sustainability. Front Bioeng Biotechnol. 2019;7:24. https://doi.org/10.3389/fbioe.2019.
00024
27. Lau SE, Teo WFA, Teoh EY, Tan BC. Microbiome engineering and plant biostimulants for sustainable crop improvement and mitigation of biotic and abiotic stresses. Discov Food. 2022;2:9. https://doi.org/10.1007/s44187-022-00009-5
28. Goulet BE, Roda F, Hopkins R. Hybridization in plants: Old ideas, new techniques. Plant Physiol. 2017;173(1):65–78. https://doi.org/
10.1104/pp.16.01340
29. Kristensen TN, Hoffmann AA, Pertoldi C, Stronen AV. What can livestock breeders learn from conservation genetics and vice versa? Front Genet. 2015;6:38. https://doi.org/10.3389/fgene.2015.00038
30. Gao F, Keinan A. Explosive genetic evidence for explosive human population growth. Curr Opin Genet Dev. 2016;41:130–139. https://doi.org/10.1016/j.gde.2016.09.002
31. Dethlefsen L, McFall-Ngai M, Relman DA. An ecological and evolutionary perspective on human–microbe mutualism and disease. Nature. 2007;449(7164):811–818. https://doi.org/10.103
8/nature06245
32. Kethireddy S, Kumar A. Infections in the immunosuppressed and immunocompromised. In: Roberts P, Todd SR, editors. Comprehensive Critical Care Review: Adult. Mount Prospect: SCCM Publications; 2012. p. 461–490.
33. Yoshida K, Schuenemann VJ, Cano LM, Pais M, Mishra B, Sharma R, et al. The rise and fall of the Phytophthora infestans lineage that triggered the Irish potato famine. Elife. 2013;2:00731. https://doi.o
rg/10.7554/eLife.00731
34. Cieslak TJ, Kortepeter MG, Wojtyk RJ, Jansen HJ, Reyes RA, Smith JO. Beyond the dirty dozen: A proposed methodology for assessing future bioweapon threats. Mil Med. 2018;183(1-2):59–65. https://doi.org/10.1093/milmed/usx004
35. Stokes HW, Gillings MR. Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens. FEMS Microbiol Rev. 2011;35(5):790–819. https://doi.
org/10.1111/j.1574-6976.2011.00273.x
36. Morens DM, Fauci AS. Emerging infectious diseases: Threats to human health and global stability. PLoS Pathog. 2013;9(7):1003467. https://doi.org/10.1371/journal.ppat.100346
7
37. Fan Y, Moon JJ. Particulate delivery systems for vaccination against bioterrorism agents and emerging infectious pathogens. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2017;9(1):e1403. https://doi.org/10.1002/wnan.1403
38. Molodysky E, Grant R. Person-to-person cancer transmission via allogenic blood transfusion. Asian Pac J Cancer Prev. 2021;22(3):641–649. https://doi.org/10.31557/APJCP.2021.22.3.
641
39. Greenhall GHB, Ibrahim M, Dutta U, Doree C, Brunskill SJ, Johnson RJ, et al. Donor-transmitted cancer in orthotopic solid organ transplant recipients: A systematic review. Transpl Int. 2022;35:10092. https://doi.org/10.3389/ti.2021.10092
40. Curd EE, Hart SFM, Lubkowitz J, Tracy KM, Milazzo L, Bodnar M, et al. Brown bullhead catfish melanoma represents a novel transmissible cancer. Nature. 2026. https://doi.org/10.1038/s4158
6-026-10828-6
41. Wiles S. All models are wrong, but some are useful: Averting the “microbial apocalypse.” Virulence. 2015;6(8):730–732. https://doi.
org/10.1080/21505594.2014.1001238
42. Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268–281. https://doi.org/10.1111/j.1469-0691.
2011.03570.x
43. Sharma A, Farouk IA, Lal SK. COVID-19: A review on the novel coronavirus disease evolution, transmission, detection, control and prevention. Viruses. 2021;13(2):202. https://doi.org/10.3390/v130
20202
44. Hart CA, Beeching NJ. A spotlight on anthrax. Clin Dermatol. 2002;20(4):365–375. https://doi.org/10.1016/S0738-081X(02)00
256-0
45. Gräser Y, Kühnisch J, Presber W. Molecular markers reveal exclusively clonal reproduction in Trichophyton rubrum. J Clin Microbiol. 1999;37(11):3713–3717. https://doi.org/10.1128/JCM.
37.11.3713-3717.1999
46. Cullen DW, Toth IK, Pitkin Y, Boonham N, Walsh K, Barker I, et al. Use of quantitative molecular diagnostic assays to investigate Fusarium dry rot in potato stocks and soil. Phytopathology. 2005;95(12):1462–1471. https://doi.org/10.1094/PHYTO-95-146
2
47. Pulcini C. Antibiotic stewardship: Update and perspectives. Clin Microbiol Infect. 2017;23(11):791–792. https://doi.org/10.1016/j.
cmi.2017.08.020
48. Mackenbach JP. Global environmental change and human health: A public health research agenda. J Epidemiol Community Health. 2007;61(2):92–94. https://doi.org/10.1136/jech.2005.045211
49. O’Gorman CM. Airborne Aspergillus fumigatus conidia: A risk factor for aspergillosis. Fungal Biol Rev. 2011;25(3):151–157. https://doi.
org/10.1016/j.fbr.2011.07.002
50. Lipsitch M. If a global catastrophic biological risk materializes, at what stage will we recognize it? Health Secur. 2017;15(4):331–334. https://doi.org/10.1089/hs.2017.0037
51. Rocha EMF, Garcia-Effron G, Park S, Perlin DS. A Ser678Pro substitution in Fks1p confers resistance to echinocandin drugs in Aspergillus fumigatus. Antimicrob Agents Chemother. 2007;51(11):4174–4176. https://doi.org/10.1128/AAC.00917-07
52. English BK, Gaur AH. The use and abuse of antibiotics and the development of antibiotic resistance. Adv Exp Med Biol. 2010;659:73–82. https://doi.org/10.1007/978-1-4419-0981-7_6
53. Balajee SA, Borman AM, Brandt ME, Cano J, Cuenca-Estrella M, Dannaoui E, et al. Sequence-based identification of Aspergillus, Fusarium, and Mucorales species in the clinical mycology laboratory: Where are we and where should we go from here? J Clin Microbiol. 2009;47(4):877–884. https://doi.org/10.1128/JCM.016
85-08
54. Miedaner T, Geiger HH. Biology, genetics, and management of ergot (Claviceps spp.) in rye, sorghum, and pearl millet. Toxins. 2015;7(3):659–678. https://doi.org/10.3390/toxins7030659
55. Anderson K, Morris G, Kennedy H, Croall J, Michie J, Richardson MD, et al. Aspergillosis in immunocompromised paediatric patients: Associations with building hygiene, design, and indoor air. Thorax. 1996;51(3):256–261. https://doi.org/10.1136/thx.51.3.256
56. Ryan CP. Zoonoses likely to be used in bioterrorism. Public Health Rep. 2008;123(3):276–281. https://doi.org/10.1177/0033354908
12300308
57. Goudoudaki S, Kambouris ME, Manoussopoulou M, Patrinos GP, Velegraki A, Manoussopoulos Y. Fast and sustainable thermo-osmotic DNA extraction protocol for trans-spectrum contingency and field use. Bio Protoc. 2023;13(17):4796. https://doi.org/10.21
769/BioProtoc.4796
58. Leiser OP, Hobbs EC, Sims AC, Korch GW, Taylor KL. Beyond the list: Bioagent-agnostic signatures could enable a more flexible and resilient biodefense posture than an approach based on priority agent lists alone. Pathogens. 2021;10(11):1497. https://doi.org/10.
3390/pathogens10111497
59. Kambouris ME, Pavlidis C, Skoufas E, Arabatzis M, Kantzanou M, Velegraki A, et al. Culturomics: A new kid on the block of OMICS to enable personalized medicine. OMICS. 2018;22(2):108–118. https://doi.org/10.1089/omi.2017.0017
60. Kambouris ME, Manoussopoulos Y, Velegraki A, Patrinos GP. The biote-bot hybrid. The ultimate biothreat merging nanobots, AI-enabled cybernetics and synthetic biology. Future Med AI. 2023;1(1):FMAI4. https://doi.org/10.2217/fmai-2023-0008
61. Shuwen H, Yifei S, Xinyue W, Zhanbo Q, Xiang Y, Xi Y. Advances in bacteria-based drug delivery systems for anti-tumor therapy. Clin Transl Immunology. 2024;13(7):1518. https://doi.org/10.1002/cti
2.1518
62. Zhang HK, Xu BW, Jia ZY, Li B, Feng XQ. Inverse design of three-dimensional multicellular biobots with target functions. J Mech Phys Solids. 2024;187:105634. https://doi.org/10.1016/j.jmps.2024.10
5634
63. Iruzubieta P, Lazarus JV, Crespo J. SARS-CoV-2 detection by self-testing: A method to improve surveillance programmes. Gastroenterol Hepatol (Engl Ed). 2021;44(6):395–397. https://doi.
org/10.1016/j.gastre.2021.01.001
64. Ludovici GM, Gabbarini V, Cenciarelli O, Malizia A, Tamburrini A, Pietropaoli S, et al. A review of techniques for the detection of biological warfare agents. Def. S&T Tech. Bull. 2016;8(1):17–26.
65. Kambouris ME, Siamoglou S, Kordou Z, Milioni A, Vassilakis S, Goudoudaki S, et al. Point-of-need molecular processing of biosamples using portable instrumentation to reduce turnaround time. Biosaf Health. 2020;2(3):177–182. https://doi.org/10.1016/j.
bsheal.2020.06.001
66. Gargis AS, Cherney B, Conley AB, McLaughlin HP, Sue D. Rapid detection of genetic engineering, structural variation, and antimicrobial resistance markers in bacterial biothreat pathogens by nanopore sequencing. Sci Rep. 2019;9(1):13501. https://doi.org/10
.1038/s41598-019-49700-1
67. Shrestha K, Kim S, Han J, Zhang M, Parajuli S, Cho G. Infectious disease diagnostic device using rapid and efficient qPCR assays on a multi-target chip: idream-qPCR. Microsyst Nanoeng. 2025;11(1):143. https://doi.org/10.1038/s41378-025-00972-w
68. Wang X, Hong XZ, Li YW, Li Y, Wang J, Chen P, et al. Microfluidics-based strategies for molecular diagnostics of infectious diseases. Mil Med Res. 2022;9(1):11. https://doi.org/10.1186/s40779-022-0037
4-3
69. Kambouris ME. Mobile stand-off and stand-in surveillance against biowarfare and bioterrorism agents. In: Karampelas P, Bourlai T, editors. Surveillance in Action: Advanced Sciences and Technologies for Security Applications. Cham: Springer; 2018. p. 241–255. https://doi.org/10.1007/978-3-319-68533-5_12
70. Priye A, Wong S, Bi Y, Carpio M, Chang J, Coen M, et al. Lab-on-a-drone: Toward pinpoint deployment of smartphone-enabled nucleic acid-based diagnostics for mobile health care. Anal Chem. 2016;88(9):4651–4660. https://doi.org/10.1021/acs.analchem.5b
04153
71. Watts AC, Ambrosia VG, Hinkley EA. Unmanned aircraft systems in remote sensing and scientific research: Classification and considerations of use. Remote Sens. 2012;4(6):1671–1692. https://doi.org/10.3390/rs4061671
72. Ratković M, Kovačević V, Marijan M, Kostadinov M, Miljković T, Bjelić M. Drone-enabled non-invasive ultrasound method for rodent deterrence. Drones. 2026;10(2):84. https://doi.org/10.3390/drones
10020084
73. Stathoulias A, Milioni A, Kritikou S, Karmakolia A, Goudoudaki S, Siamoglou S, et al. Toward high-throughput fungal electroculturomics and new omics methodologies in 21st-century microbiology and ecology. OMICS. 2020;24(8):493–504. https://do
i.org/10.1089/omi.2020.0012
74. Delcomyn C, Bushway K, Henley M. Inactivation of biological agents using neutral oxone-chloride solutions. Environ Sci Technol. 2006;40(8):2759–2764. https://doi.org/10.1021/es052146+
75. Dutta D, Ravichandiran V, Sukla S. Virophages: Association with human diseases and their predicted role as virus killers. Pathog Dis. 2021;79(8):ftab049. https://doi.org/10.1093/femspd/ftab049
76. Mogili UR, Deepak BBVL. Review on application of drone systems in precision agriculture. Procedia Comput Sci. 2018;133:502–509. https://doi.org/10.1016/j.procs.2018.07.063
77. Ascoët S, De Waard M. Diagnostic and therapeutic value of aptamers in envenomation cases. Int J Mol Sci. 2020;21(10):3565. https://doi.org/10.3390/ijms21103565
78. Arimori T, Ikemura N, Okamoto T, Takagi J, Standley DM, Hoshino A. Engineering ACE2 decoy receptors to combat viral escapability. Trends Pharmacol Sci. 2022;43(10):838–851. https://doi.org/10.10
16/j.tips.2022.06.011
79. Schauber J, Gallo RL. Antimicrobial peptides and the skin immune defense system. J Allergy Clin Immunol. 2008;122(2):261–266. https://doi.org/10.1016/j.jaci.2008.03.027
80. Iskandar K, Murugaiyan J, Hammoudi Halat D, El Hage S, Chibabhai V, Adukkadukkam S, et al. Antibiotic discovery and resistance: The chase and the race. Antibiotics. 2022;11(2):182. https://doi.org/10.3390/antibiotics11020182
81. Kambouris ME, Gaitanis G, Manoussopoulos Y, Arabatzis M, Kantzanou M, Kostis GD, et al. Humanome versus microbiome: Games of dominance and pan-biosurveillance in the omics universe. OMICS. 2018;22(8):528–538. https://doi.org/10.1089/omi.2018.0
096
82. Mathews M, Ryan D, Hedden L, Lukewich J, Marshall EG, Gill P, et al. Surge capacity and practice management challenges of Canadian family physicians during COVID-19: A qualitative study. Hum Resour Health. 2025;23(1):13. https://doi.org/10.1186/s12960-025-00981-w
83. Loreau M, Barbier M, Filotas E, Gravel D, Isbell F, Miller SJ, et al. Biodiversity as insurance: From concept to measurement and application. Biol Rev Camb Philos Soc. 2021;96(5):2333–2354. https://doi.org/10.1111/brv.12756
84. European Food Safety Authority (EFSA), Alexandrov T, Baltusyte I, Broglia A, Calistri P, Gubbins S, et al. Sheep and goat pox: Vaccines and vaccination scenarios to control the epidemics in Greece and Bulgaria in 2025. EFSA J. 2026;24(2):9928. https://doi.org/10.290
3/j.efsa.2026.9928
85. Nogués-Bravo D, Simberloff D, Rahbek C, Sanders NJ. Rewilding is the new Pandora’s box in conservation. Curr Biol. 2016;26(3):R87–R91. https://doi.org/10.1016/j.cub.2015.12.044
86. Kambouris ME. Exotic biothreats. In: Kambouris ME, editor. Preparing for the Meta-Biothreat: Disruptive Biosecurity. Cham: Springer Nature Switzerland; 2026. p. 51–68. https://doi.org/10.10
07/978-3-032-25690-4_3
87. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013;8(11):2281–2308. https://doi.org/10.1038/nprot.2013.143
88. Aleksic B, Draghi M, Ritoux S, Bailly S, Lacroix M, Oswald IP, et al. Aerosolization of mycotoxins after growth of toxinogenic fungi on wallpaper. Appl Environ Microbiol. 2017;83(16):01001-17. https://doi.org/10.1128/AEM.01001-17
89. Rivera-de-Torre E, Rimbault C, Jenkins TP, Sørensen CV, Damsbo A, Saez NJ, et al. Strategies for heterologous expression, synthesis, and purification of animal venom toxins. Front Bioeng Biotechnol. 2022;9:811905. https://doi.org/10.3389/fbioe.2021.811905
90. Smith HO, Hutchison CA, Pfannkoch C, Venter JC. Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides. Proc Natl Acad Sci USA. 2003;100(26):15440–15445. https://doi.org/10.1073/pnas.22371
26100
91. Sahl JW, Pearson T, Okinaka R, Schupp JM, Gillece JD, Heaton H, et al. A Bacillus anthracis genome sequence from the Sverdlovsk 1979 autopsy specimens. mBio. 2016;7(5):01501-16. https://doi.or
g/10.1128/mBio.01501-16
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